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Adenylylation (addition of AMP) of a lysine residue during the Energetic Centre in the enzyme, pyrophosphate is unveiled;
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DNA ligase joins the DNA molecule covalently by catalyzing the formation of phosphodiester bonds between adjacent nucleotides.
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DNA ligase three: complexes with DNA fix protein XRCC1 to assist in sealing DNA during the process of nucleotide excision maintenance and recombinant fragments. With the all recognised mammalian DNA ligases, only ligase three has become uncovered to generally be current in mitochondria.
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The activity of E. coli DNA ligase can be Improved by DNA polymerase at the correct concentrations. Improvement only performs if the concentrations of the DNA polymerase 1 are much decrease compared to the DNA fragments being ligated.
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